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dapi ii counterstain (antifade/mount)  (Abbott Laboratories)

 
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    Abbott Laboratories dapi ii counterstain (antifade/mount)
    Interphase FISH analysis with probes generated by three different fragmentation methods. LAPC4 prostate cancer cells were subject to FISH analysis using probes prepared from non-overlapping BAC clones containing DNA sequences flanking the TMPRSS2 gene. Upstream BAC RP11-35C4 was labelled with green fluorophore and downstream BAC RP11-825A8 was labelled with red fluorophore. Nuclei were counterstained with <t>DAPI.</t> (A) FISH probes prepared by sonication. (B) FISH probes prepared using Fragmentase enzyme. (C) FISH probes prepared with restriction enzyme digestion using CviQ1/AluI.
    Dapi Ii Counterstain (Antifade/Mount), supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/counterstained+in+dapi+ii+with+antifade/dapi+ii+counterstain/pmc06308247-124-237-241
    Average 90 stars, based on 1 article reviews
    dapi ii counterstain (antifade/mount) - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "Preparation of fluorescent in situ hybridisation probes without the need for optimisation of fragmentation"

    Article Title: Preparation of fluorescent in situ hybridisation probes without the need for optimisation of fragmentation

    Journal: MethodsX

    doi: 10.1016/j.mex.2018.11.015

    Interphase FISH analysis with probes generated by three different fragmentation methods. LAPC4 prostate cancer cells were subject to FISH analysis using probes prepared from non-overlapping BAC clones containing DNA sequences flanking the TMPRSS2 gene. Upstream BAC RP11-35C4 was labelled with green fluorophore and downstream BAC RP11-825A8 was labelled with red fluorophore. Nuclei were counterstained with DAPI. (A) FISH probes prepared by sonication. (B) FISH probes prepared using Fragmentase enzyme. (C) FISH probes prepared with restriction enzyme digestion using CviQ1/AluI.
    Figure Legend Snippet: Interphase FISH analysis with probes generated by three different fragmentation methods. LAPC4 prostate cancer cells were subject to FISH analysis using probes prepared from non-overlapping BAC clones containing DNA sequences flanking the TMPRSS2 gene. Upstream BAC RP11-35C4 was labelled with green fluorophore and downstream BAC RP11-825A8 was labelled with red fluorophore. Nuclei were counterstained with DAPI. (A) FISH probes prepared by sonication. (B) FISH probes prepared using Fragmentase enzyme. (C) FISH probes prepared with restriction enzyme digestion using CviQ1/AluI.

    Techniques Used: Generated, Clone Assay, Sonication

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    dapi ii counterstain (antifade/mount)  (Abbott Laboratories)
    90
    Abbott Laboratories dapi ii counterstain (antifade/mount)
    Interphase FISH analysis with probes generated by three different fragmentation methods. LAPC4 prostate cancer cells were subject to FISH analysis using probes prepared from non-overlapping BAC clones containing DNA sequences flanking the TMPRSS2 gene. Upstream BAC RP11-35C4 was labelled with green fluorophore and downstream BAC RP11-825A8 was labelled with red fluorophore. Nuclei were counterstained with <t>DAPI.</t> (A) FISH probes prepared by sonication. (B) FISH probes prepared using Fragmentase enzyme. (C) FISH probes prepared with restriction enzyme digestion using CviQ1/AluI.
    Dapi Ii Counterstain (Antifade/Mount), supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/counterstained+in+dapi+ii+with+antifade/dapi+ii+counterstain/pmc06308247-124-237-241
    Average 90 stars, based on 1 article reviews
    dapi ii counterstain (antifade/mount) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    counterstained in dapi ii with antifade  (Abbott Laboratories)
    90
    Abbott Laboratories counterstained in dapi ii with antifade
    Interphase FISH analysis with probes generated by three different fragmentation methods. LAPC4 prostate cancer cells were subject to FISH analysis using probes prepared from non-overlapping BAC clones containing DNA sequences flanking the TMPRSS2 gene. Upstream BAC RP11-35C4 was labelled with green fluorophore and downstream BAC RP11-825A8 was labelled with red fluorophore. Nuclei were counterstained with <t>DAPI.</t> (A) FISH probes prepared by sonication. (B) FISH probes prepared using Fragmentase enzyme. (C) FISH probes prepared with restriction enzyme digestion using CviQ1/AluI.
    Counterstained In Dapi Ii With Antifade, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/counterstained+in+dapi+ii+with+antifade/counterstained+in+dapi+ii+with+antifade/pm20688391-120-24-28
    Average 90 stars, based on 1 article reviews
    counterstained in dapi ii with antifade - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Interphase FISH analysis with probes generated by three different fragmentation methods. LAPC4 prostate cancer cells were subject to FISH analysis using probes prepared from non-overlapping BAC clones containing DNA sequences flanking the TMPRSS2 gene. Upstream BAC RP11-35C4 was labelled with green fluorophore and downstream BAC RP11-825A8 was labelled with red fluorophore. Nuclei were counterstained with DAPI. (A) FISH probes prepared by sonication. (B) FISH probes prepared using Fragmentase enzyme. (C) FISH probes prepared with restriction enzyme digestion using CviQ1/AluI.

    Journal: MethodsX

    Article Title: Preparation of fluorescent in situ hybridisation probes without the need for optimisation of fragmentation

    doi: 10.1016/j.mex.2018.11.015

    Figure Lengend Snippet: Interphase FISH analysis with probes generated by three different fragmentation methods. LAPC4 prostate cancer cells were subject to FISH analysis using probes prepared from non-overlapping BAC clones containing DNA sequences flanking the TMPRSS2 gene. Upstream BAC RP11-35C4 was labelled with green fluorophore and downstream BAC RP11-825A8 was labelled with red fluorophore. Nuclei were counterstained with DAPI. (A) FISH probes prepared by sonication. (B) FISH probes prepared using Fragmentase enzyme. (C) FISH probes prepared with restriction enzyme digestion using CviQ1/AluI.

    Article Snippet: Tryptone (ThermoFisher Scientific, Scoresby, VIC, AUS) Yeast Extract (ThermoFisher Scientific, Scoresby, VIC, AUS) Bacto Agar (ThermoFisher Scientific, Scoresby, VIC, AUS) Chloramphenicol (SIGMA, Castle Hill, NSW, AUS) TRIS (SIGMA, Castle Hill, NSW, AUS) EDTA (SIGMA, Castle Hill, NSW, AUS) RNAse (SIGMA, Castle Hill, NSW, AUS) SDS (SIGMA, Castle Hill, NSW, AUS) NaOH (SIGMA, Castle Hill, NSW, AUS) Guanidine Hydrochloride (GuHCl) (SIGMA, Castle Hill, NSW, AUS) Potassium Acetate (KAc) (SIGMA, Castle Hill, NSW, AUS) Potassium Chloride (SIGMA, Castle Hill, NSW, AUS) Sodium Chloride (SIGMA, Castle Hill, NSW, AUS) Trisodium citrate dihydrate (Na 3 Citrate ⋅ 2H 2 O) (SIGMA, Castle Hill, NSW, AUS) NP40 (SIGMA, Castle Hill, NSW, AUS) Acetic acid, glacial (Chem-supply, Gilman, SA, AUS) Isopropanol (Chem-supply, Gilman, SA, AUS) Ethanol (Chem-supply, Gilman, SA, AUS) QIAGENTM plasmid mini kit (QIAGEN, Chadstone, VIC, AUS) CviQI (Genesearch, NEB, Arundel, QLD, AUS) AluI (Genesearch, NEB, Arundel, QLD, AUS) Phenol (SIGMA, Castle Hill, NSW, AUS) Chloroform (SIGMA, Castle Hill, NSW, AUS) Sodium Acetate trihydrate (NaAc) (SIGMA, Castle Hill, NSW, AUS) Glycogen (Ambion, ThermoFisher Scientific, Scoresby, VIC, AUS, cat#9510, 5 mg/ml) AMPure XP beads (Beckmann Coulter, Lane Cove, NSW, AUS) KREATECH ULSTM platinum bright labelling kit (Diagnostic Technology, Belrose, NSW, AUS) BIORAD P30 SSC spin columns (BIORAD, Gladesville, NSW) C0t1 DNA (species specific) (Life Technologies, ThermoFisher Scientific, Scoresby, VIC, AUS, 500 μg @ 1 mg/ml Cat# 15279-011 – for human) Hybridisation buffer Vysis LSI/WCP hybridisation buffer (Abbott, North Ryde, NSW, AUS, Cat# 06J67-001) DAPI II counterstain (antifade/mount) (Abbott, North Ryde, NSW, AUS, Cat# 06J50-001)

    Techniques: Generated, Clone Assay, Sonication